Rat Cyclic Guanosine Monophosphate (cGMP) ELISA Kit

Cat.#:

JL11179

Regulatory Status:

RUO

Alternative:

c-GMP; 3';5'-Cyclic GMP; Cyclic 3';5'-GMP; Guanosine 3';5'-Cyclic Phosphate

Detection Method:

ELISA

Assay Type:

Competitive

Detection Range:

0.156-10ng/mL

Sensitivity:

0.08ng/mL

Specificity:

This kit recognizes Rat cGMP in samples. No significant cross-reactivity or interference between Rat cGMP and analogues was observed.

Sample Type:

Serum;plasma;tissue homogenates;cell lysates;cell culture supernates and other biological fluids

Assay Procedure:

Research Area:

Signal Transduction

Size & Price:

Human A1BG Polyclonal Antibody

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Product Datasheets

Instruction:

Data
Citations
Sequence / 3D

| cGMP

Cyclic guanosine monophosphate (cGMP) is a cyclic nucleotide derived from guanosine triphosphate (GTP). cGMP acts as a second messenger much like cyclic AMP.Through protein kinases activation, cGMP can relax smooth muscle.cGMP concentration in urine can be measured for kidney function and diabetes detection.Guanylate cyclase (GC) catalyzes cGMP synthesis. This enzyme converts GTP to cGMP.

| Typical data

As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, wash technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data are provided below for reference only.

Concentration (ng/mL)	10	5	2.5	1.25	0.625	0.312	0.156	0
OD	0.216	0.298	0.417	0.669	0.955	1.405	1.79	1.865

| Repeatability

Intra-assay Precision (Precision within an assay)：CV% < 10%; Inter-assay Precision (Precision between assays)：CV% < 10%

| Recovery

The recovery of Rat cGMP spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range(%)	Average Recovery(%)
Serum(n=8)	81-101	96
Plasma(n=8)	92-105	101
Cell Culture Supernates(n=8)	97-108	105

| Linearity

Samples were spiked with high concentrations of Rat cGMP and diluted with Universal Diluent to produce samples with values within the range of the assay.

		Serum(n=4)	Plasma(n=4)	Cell Culture Supernates(n=4)
1 : 2	Range (%)	83-95	88-96	90-110
1 : 2	Average (%)	91	93	96
1 : 4	Range (%)	89-104	87-108	105-115
1 : 4	Average (%)	93	98	110